Applications
The Oroboros technology for high-resolution respirometry (HRR) in mitochondria and cell research:
The Oroboros allows the measurement of respiration at controlled oxygen levels, combined with redox biology (NADH and CoQ), ROS production, mitochondrial membrane potential, ATP production, Ca2+, or pH. HRR expands to high-resolution PhotoBiology.
Applications:
- O2 consumption
- Normoxia-Hyperoxia-Hypoxia-Anoxia
- Q-redox state
- NAD(P)H-redox state
- H2O2 production
- mt-Membrane potential
- ATP production
- Ca2+ concentration
- pH
- Photosynthesis
- Dark respiration
With the Oroboros, small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissues slices.
Experimental data are displayed not only as oxygen concentration over time but also as real-time transformation of these data, allowing the experimenter to follow respiration (oxygen consumption, oxygen flux) of biological and biochemical samples while the experiment is still running.
