NextGen-O2k Redox

Configuration

NextGen-O2k: Hardware and DatLab Software*

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 Sockets for Q-and NAD(P)H-Modules
2 Sockets for PB-Module
2 O2k-Chambers (2.0 mL, Duran® glass)
2 SmartPOS
2 stirrers (white PVDF) , electromagnetic control
2 stoppers (black PEEK) with O-ring
2 O2k-Chamber Holders (blue POM) with O-ring and V-ring
2 POS-Holders (blue POM)

Current DatLab version with free follow-up upgrades within the warranty period

NextGen-O2k: Accessories and Consumables                                                                                                  

1 ISS-Integrated Suction System, to remove medium from the O2k-Chamber
1 Oxia – the O2 regime controller for HyperOxia and HypOxia
1 O2k-Titration Set: Hamilton microsyringes; fixed needles with rounded tips (8 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 2 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 Pkg. POS-Seal Tip
2 Pkg. POS-Membranes
1 POS-Electrolyte Powder
1 POS-Mounting Tool
1 Polishing Cloth
1 Polishing Powder 0.3 μm
1 O2-Zero Powder
1 Forceps for membrane application
1 Pipette Plastic ungraded
1 Pkg. Conditioning cloths for stainless-steel housing

O2k-Q-Module: for measuring the Q-redox state and cyclic voltammetry

4  Q-Stoppers (beige PEEK conical shaft side+2.6 mm +1.3 mm central port): with glassy carbon and platinum electrode embedded
2 Reference electrodes, 2.4mm Ø glass shaft
10 Replacement barrels for reference electrodes , 2.4 mm glass
1 Electrolyte Reference-Electrode
1 ISE-Filling Syringe with needle
1 Stopper-Needle
2 Polishing Powder 0.3 μm
2 Polishing Powder 0.05 µm
4 Polishing Cloth
3 Pkgs Q-Filter Papers (10/Pkg)
2 Boxes O-ring Viton® 12.5×1 mm, 8/box

O2k-NADH-Module: for simultaneous measurement of oxygen consumption and NAD(P)H autofluorescence.

2 NADH-Sensors
2 stirrers (black PVDF)
3 Pkg of Filter sets R370 for NADH (12/Pkg)

O2k-TIP2k-Module: add-on for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control

4 Micro-injection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 O2k-Chambers-sV (Duran® glass), with smaller operation volume (V) of 0.5 mL
2 stirrers sV (white PVDF) for small volume chambers, electromagnetic control
2 stoppers sV (black PEEK) for small volume chambers with O-ring
2 O2k-Chamber Holders sV (black POM) with O-ring and V-ring
2 POS-Holders sV (black POM)

Included extras                                                                                           

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 Installation and startup support session (remote, to be used within the warranty period)
1 O2k-Workshop registration fee for the participation of one lab member waived (to be used within the warranty period)
3-year warranty
* Integrated computer with touchscreen connects DatLab to the NextGen-O2k
* Keyboard set, webcam and webcam accessories

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